Polyribosome isolation in the presence of diethyl pyrocarbonate.
نویسندگان
چکیده
Isolation of polyribosomes from wheat embryos and corn root tips in the presence of diethyl pyrocarbonate showed this reagent to have a protective effect on polyribosome structure. In addition, the use of diethyl pyrocarbonate allowed initial homogenization to be performed under less stringent conditions than those normally employed for polyribosome isolation. The use of the reagent is however limited, in that it is deleterious to in vitro ribosomal amino acid incorporation.
منابع مشابه
Polyribosome Isolation in the Presence of Diethyl Pyrocarbonatel
Isolation of polyribosomes from wheat embryos and corn root tips in the presence of diethyl pyrocarbonate showed this reagent to have a protective effect on polyribosome structure. In addition, the use of diethyl pyrocarbonate allowed initial homogenization to be performed under less stringent conditions than those normally employed for polyribosome isolation. The use of the reagent is however ...
متن کاملPolyribosomes from Peas AN IMPROVED METHOD FOR THEIR ISOLATION IN THE ABSENCE OF RIBONUCLEASE INHIBITORS ' Received for publication
Profiles of polyribosomes were obtained from etiolated stem segments of Pisum sativum L. var. Alaska isolated in various buffers. Tissue homogenized in a medium containing 0.2 M tris-HCI, pH 8.5, 0.2 M sucrose, 30 mM MgCL, and 60 mM KCI yielded polyribosomes exhibiting far less degradation than tissue homogenized in conventional media containing tris-HCl at lower ionic strength and pH. A furthe...
متن کاملReaction of diethyl pyrocarbonate with nucleic acid components. I. Adenine.
The use of diethyl pyrocarbonate as a nuclease inhibitor in the preparation of RNA of high molecular weight has prompted a study of the possible reactions of this compound with nucleic acid components under the conditions generally employed for providing inhibition. The first substrate investigated was adenine, which has been found to undergo ring opening with the formation of 5(4)-N-carbethoxy...
متن کاملChemical and kinetic evidence for an essential histidine in the phosphotriesterase from Pseudomonas diminuta.
The pH rate profile for the hydrolysis of diethyl-p-nitrophenyl phosphate catalyzed by the phosphotriesterase from Pseudomonas diminuta shows a requirement for the deprotonation of an ionizable group for full catalytic activity. This functional group has an apparent pKa of 6.1 +/- 0.1 at 25 degrees C, delta Hion of 7.9 kcal/mol, and delta Sion of -1.4 cal/K.mol. The enzyme is not inactivated in...
متن کاملHoogsteen base pairs proximal and distal to echinomycin binding sites on DNA.
Forms of the DNA double helix containing non-Watson-Crick base-pairing have been discovered recently based on x-ray diffraction analysis of quinoxaline antibiotic-oligonucleotide complexes. In an effort to find evidence for Hoogsteen base-pairing at quinoxaline-binding sites in solution, chemical "footprinting" (differential cleavage reactivity) of echinomycin bound to DNA restriction fragments...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Plant physiology
دوره 44 9 شماره
صفحات -
تاریخ انتشار 1969